Mätning utfördes på BD Accuri ™ C6 Cytometer. Analys och Protein concentration was determined by BCA assay kit according to user's manual. 10–20 A total of 20.000 ungated events were acquired on an Accuri C6 Plus Flow Cytometer.

Cells were analyzed using a BD Accuri™ C6 (BD Biosciences) flow cytometer and analyzed using the BD Accuri™ C6 software. .. Soft-agar assay 104 cells were plated in 60mm dishes in a solution containing Dulbecco's modified Eagle's medium 2× (Sigma, St Louis, MO, USA), TPB Buffer (Difco, BD, Franklin Lakes, NJ, USA), and 1.25% of Noble Agar (Difco, BD, Franklin Lakes, NJ, USA). BD Accuri™ C6 default optics Detector FL1 FL2 FL3 FL4 Filter 533/30nm 585/40nm 670nm LP 675/25nm Fluorochrome FITC PE PerCP, PerCP-Cy5.5 APC Color Green Yellow/Orange Dark Red Red “3 blue 1 red” Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Accuri C6 is a digital PC based bench top analyser, 2 laser system. Standard fluorochrome in use are identical to FACS Calibur. Even more colors to choose from with selectable lasers and filter replacement.

BD Accuri C6 with sample volumes as low as 300 μL (assuming a volume run limit of 10 μL) in BD Falcon™ 12 x 75-mm tubes. The maximum recommended sample volume in a 12 x 75-mm tube is 2 mL. Never acquire more than 750 μ L from a single tube using the Medium fluidics setting, or 1,500 μ L using the Fast Deionised water is recommended in the C6 manual - try this and it may stop blockages. Many thanks, Matt . Cite. 28th Feb, 2020. Anyone have experience with the new BD Accuri C6 Plus?

Anyone have experience with the new BD Accuri C6 Plus? Item BD Accuri™ C6 Plus Flow Cytometer; Company BD Biosciences; Catalog Number BD Accuri C6 Plus; This product is no longer available on Biocompare.

BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Turn on the computer and log in with your UCF info. Start the Accuri CSampler software.

To fill the cleaning solution bottle (green line), add 3 ml of 10-30% citric acid and fill with DI. There are two options here: Buy from Accuri, or make your own citric acid solution. It is cheaper and easier to make your own. BD Accuri C6 with plate handler acquisition April 2016 Multi-well Auto Collect sample Important – only run this protocol after you have performed “Cytometer Setup/Verification” protocol on your BD Accuri C6 cytometer using particles.

BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. BD Accuri C6 Software will display this notification based on whichever trigger is reached first. Page 19: Decontamination Fluid Cycle 6.

All current service contracts will be honored. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer.

BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. BD Accuri C6 Software will display this notification based on whichever trigger is reached first. Page 19: Decontamination Fluid Cycle 6. BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. BD Accuri™ C6 Flow Cytometer Quick Set Up Guide Please follow these directions for setting up your BD Accuri C6 Flow Cytometer.
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Install BD CSampler software. 10. Turn on the BD Accuri C6. 11. Run validation beads to ensure correct operation.

Standard fluorochrome in use are identical to FACS Calibur. Even more colors to choose from with selectable lasers and filter replacement. Available to trained users with online booking. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform.
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BD Accuri™ C6 Flow Cytometer Quick Set Up Guide Please follow these directions for setting up your BD Accuri C6 Flow Cytometer. If you have questions or require further clarification, see the BD Accuri C6 Flow Cytometer Instrument Manual or contact Accuri Technical Support.

Click the link below to read about the impactful work done by the small champion, BD Accuri™. Click here to view the publications on Google Scholar. Class 1 Laser Product. For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD Biosciences Accuri C6 Plus. Instrument Description: Flow Cytometry Analyzer.

Introducing the BD Accuri™ C6 Plus. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform.

595,00 30-0100. GIVI MONORACK UNIVERSAL F. 1 295,00. 1 036,00. 1. C6. 0. 30-0200. Grafisk manual Mallar Utveckling Den klassiska Göteborgsblå är den färg som starkast Grafisk manual.

Place the mat in position (Figure 10). WARNING Failure to keep the mat clear of plates, tubes or any other materials may result in damage to the BD CSampler. 9. Install BD CSampler software.