This postgermination developmental checkpoint is signaled by the stress hormone abscisic acid (ABA), which induces the expression of the bZIP transcription activator ABI5. The growth arrest efficiency depends on ABI5 levels, and abi5 mutants are ABA-insensitive and …

The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression. Our findings reveal a novel transcriptional switch, composed of JMJ17–WRKY40 and HY5–ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis.

As a ABI5 is a key component in ABA-triggered pathways during germination, seedling establishment, and vegetative growth (Lopez-Molina et al., 2001); in addition, it has been reported to play certain roles in nitrogen assimilation and signaling (Signora et al., 2001). In the ABI5 clade of the bZIP transcription factors, several ABA-RESPONSIVE ELEMENT-BINDING FACTORS (abbreviated as either AREBs or ABFs) share several motifs with ABI5 in addition to the conserved bZIP domain (Jakoby et al., 2002). The Arabidopsis abscisic acid (ABA)–insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. mutants, the abi5-1 mutants had lower photosynthetic rates and Fv/Fm compared to the wild type under photorespiratory conditions i.e.

ABA Stabilizes ABI5. Given the strong ABA-dependent ABI5 protein accumulation, we were interested to determine whether ABI5 protein stability is altered in the presence of ABA. For this purpose we generated WT/35S∷HA-ABI5 transgenic lines constitutively expressing HA-ABI5 transcript. Without ABA, FT expression was significantly upregulated in abi5-7 plants. Contrarily, with ABA treatment the level of FT expression was drastically decreased in abi5-7 but not in Col or ABI5 S42AS145AT201A plants. In addition, FT expression was sustained at lower level with or without ABA treatment in ABI5-5 plants.

PLoS Genetics. Vol. 10 (2), p. artikel nr De GA och ABA signalering vägar är sammanflätade, som ett uttryck för vissa En värdefull strategi för att förstå rollen som vissa aktörer i GA/ABA seed germination by stimulating abscisic acid synthesis and ABI5 activity.

De GA och ABA signalering vägar är sammanflätade, som ett uttryck för vissa En värdefull strategi för att förstå rollen som vissa aktörer i GA/ABA seed germination by stimulating abscisic acid synthesis and ABI5 activity.

Responsible for reducing cadmium uptake, mediated by interaction with MYB49 . Other Gene Models The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression. Our findings reveal a novel transcriptional switch, composed of JMJ17-WRKY40 and HY5-ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis.

Convergence of Light and ABA Signaling on the ABI5 Promoter Dongqing Xu1,2., Jigang Li2,3,4., Sreeramaiah N. Gangappa1¤a, Chamari Hettiarachchi1¤b, Fang Lin2, Mats X. Andersson1, Yan Jiang1, Xing Wang Deng2,4*, Magnus Holm1{ 1Department of Biological and Environmental Sciences, Gothenburg University, Gothenburg, Sweden, 2Peking-Yale Joint Center for Plant Molecular Genetics …

Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. ABI5 HY5 Light ABA BBX21 ABI5 PYR/PYL/RCAR PP2C SnRK2 COP1 FHY3 DET1 +1 HRB2 HDA6 ABI5 ABI5 HRB2 HDA6 DDA1 ABA-response genes PIF1,3,4,5 PIF1,3,4,5 Fig.1 A model showing molecular interactions between light and abscisic acid (ABA) signalling pathways during … The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination … 2019-08-09 2015-03-20 2018-02-20 negative regulator of ABA signaling that promotes ABI5 degradation during seed germination. Over-expression of AFP1 enhances tolerance to ABA and reduces ABI5 accumulation, whereas afp1 mutants are sensitive to ABA and have high levels of ABI5 (Lopez-Molinaetal.,2003).TherearethreeAFP1homologs(i.e. AFP2, AFP3, and AFP4) in the Arabidopsis genome, 2001-04-10 Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages. Responsible for reducing cadmium uptake, mediated by interaction with MYB49 . Further, we measured JA concentrations in Col‐0 and abi5‐7 with or without ABA treatment.

Further studies are needed to determine the physiological function of the ABA pathway in mediating nitrogen availability and disrupted C/N stress. 2014-02-27 · ABI5 expression is greatly reduced in the abi3 mutants, and 35S:ABI5 could rescue ABA insensitivity of abi3.
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Tidigare arbete har visat att i vilande frön fröskalet syntetiserar och frigör ABA mot seed germination by stimulating abscisic acid synthesis and ABI5 activity. ABI5. A. thaliana (Columbia). GIA1, F2H17.12, F2H17_12, AT2G36270, ABA INSENSITIVE 5, GROWTH-INSENSITIVITY TO ABA more, protein abscisic Together, these data suggest that ATHB5 acts as a regulator of seed germination and postgerminative growth downstream of ABI3 and ABI5 in an ABA Andra med liknande namn.

negative regulator of ABA signaling that promotes ABI5 degradation during seed germination.
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Beyond germination, ABA further inhibits postgermination seedling development, which is often mediated by the transcription factor ABSCISIC ACID INSENSTIVE5 (ABI5) (Chen et al., 2020). Recent investigations have unravelled the importance of light–ABA interactions in postgermination development and environmental adaptability of seedlings.

Compared with ABI5 accumulation in WT seeds, ABI5 accumulation was higher in abt-2 but lower in abi2-1C and abt-2abi2-1C (Supplemental Figure 5).

We used cDNA microarrays to analyze ABA regulated gene expression and the role of ABI5 in seedlings. 310 genes were identified as. ABI5/ABA regulated

2016-09-14 · Furthermore, 35S:ABI5 dramatically attenuated or abolished the ABA insensitivity of nf-ycT and rgl2, while abi5 35S:NF-YC9 still remained high testa and endosperm rupture rates as abi5. ABA-responsive through ABI5-dependent signaling (e.g., RD29A, Rd29B, AtEm6, RAB18, ADH1) was hyperinduced by the hormone in siz1 seedlings. abi5–4 suppressed ABA hypersensitivity caused by siz1 (siz1–2 abi5–4), demonstrating an epistatic genetic inter-action between SIZ1 and ABI5. A K391R substitution in ABI5 2019-12-02 · Since XIW1 affects ABI5 protein abundance, and both XIW1 and ABI5 are located in the nucleus in the presence of ABA, we wondered whether there is a direct interaction between XIW1 and ABI5. Yeast two-hybrid assays showed that XIW1 directly interacts with ABI5 through its WD40 domains ( Figure 7 A ). negative regulator of ABA signaling that promotes ABI5 degradation during seed germination. Over-expression of AFP1 enhances tolerance to ABA and reduces ABI5 accumulation, whereas afp1 mutants are sensitive to ABA and have high levels of ABI5 (Lopez-Molinaetal.,2003).TherearethreeAFP1homologs(i.e.

This subfamily has a broader consensus‐binding site than the other bZIP proteins in that its members tolerate variability in the ACGT core element essential to the ABRE G‐box ( Kim et al ., 1997 ). 2013-01-09 2013-01-18 2017-12-01 2016-09-14 2009-03-31 This ABA‐mediated developmental checkpoint requires the bZIP transcription factor ABI5. Here, we used abi3‐1, which is also unable to execute this checkpoint, to investigate the relative role of ABI3 and ABI5 in this process. In wild‐type Arabidopsis plants, ABI3 expression and activity parallel those described for ABI5 following Convergence of Light and ABA Signaling on the ABI5 Promoter Dongqing Xu1,2., Jigang Li2,3,4., Sreeramaiah N. Gangappa1¤a, Chamari Hettiarachchi1¤b, Fang Lin2, Mats X. Andersson1, Yan Jiang1, Xing Wang Deng2,4*, Magnus Holm1{ 1Department of Biological and Environmental Sciences, Gothenburg University, Gothenburg, Sweden, 2Peking-Yale Joint Center for Plant Molecular Genetics … 2002-11-01 ABI5 encodes a bZIP transcription factor, and is domi-nantly expressed in seeds but not in vegetative tissues, indicating that these transcription factors specifically function in seed mat-uration and germination.12,13 Like drought and high-salt stress, exogenous application of ABA induces ABI5 expression in germi - ABA-responsive through ABI5-dependent signaling (e.g., RD29A, Rd29B, AtEm6, RAB18, ADH1) was hyperinduced by the hormone in siz1 seedlings. abi5–4 suppressed ABA hypersensitivity caused by siz1 (siz1–2 abi5–4), demonstrating an epistatic genetic inter-action between SIZ1 and ABI5.